Nest Scientific
NEST GelNest Matrix, for hESC Culture, with Phenol Red, LDEV-Free, 5 mL/bottle, 1 bottle/bag - 211272
GelNest™ is a basement membrane product identical in composition and performance to Corning® Matrigel® Matrix. GelNest™ Matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcomas using genetically identical mouse lines and SOP's. EHS tumors contain plentiful ECM proteins such as laminin (60% ), collagen IV (30%), heparan sulfate proteoglycans, nidogen (8%), and several growth factors.
Part number 211272 is a human embryonic stem cell (hESC) qualified product. It has undergone additional quality control testing to ensure that hESCs grown on this matrix for five passages remain undifferentiated based on standard morphology criteria and surface markers. It also feature a reduced concentration of growth factors to prevent unwanted cell differentiation.
Equivalent to Corning:
- 354277
Please note this formulation contains phenol red as an indicator. When frozen the color may differ from yellow to dark red. This is normal and results from the interaction of carbon dioxide, the bicarbonate buffer and phenol red. The color variation is harmless and goes away when the product reaches temperature and carbon dioxide equilibrium in the incubator.
Quality is guaranteed, each lot is produced and tested under stringent quality controls:
- Mouse colonies regularly tested for pathogens using mouse antibody production (MAP) protocol
- Murine Engelbreth-Holm-Swarm (EHS) tumors used guaranteed free of LDEV
- Per lot PCR testing for a wide variety of pathogens including LDEV
- Tested and lot certified free of bacteria, fungi, and mycoplasma
- Lowry method used to determine protein concentrations of >8mg/mL per lot
- Limulus Amoebocyte Lysate assay used to quantify endotoxins, guaranteed <4EU/mL
- Gel tested for 14 days at 37°C to guarantee stability
- Lot tested using for biological activity using neurite growth and organoid formation assays
